Phis2.1 vector

Author: wgug

August undefined, 2024

WebbpYES2的是一个5.9 kb的载体，设计用来在酿酒酵母（Saccharomyces cerevisiae）中诱导表达重组蛋白。. 载体的特点在于基因插入载体的构建简单，以及能够使用原养型尿嘧啶进 … Webb16 feb. 2024 · Two GA-inducible genes expansin 1 (PhEXP1) and cysteine proteinase 1 (PhCP1) were down-regulated in the flowers infected with TRV-PhCHS/GA20ox3 . …

PT3705-5 (PR32049) pHIS2

WebbpHis2载体价格650元，pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文，载体质粒抗性为Kanamycin，质粒大小为7207bp。pHis2是酵母单杂交载体。 Webb26 maj 2024 · pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for … grand forks public school

【Plant Physiol】在高钾状态下，香豆素的积累可提高苹果对腐烂 …

WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog No. 630304 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. … Webb11 juli 2014 · 常用载体及通用测序引物,测序通用引物,华大测序通用引物,载体通用引物,petduet 1测序引物,通用引物,测序引物,pet28a测序引物,m13通用引物,细菌通用引物,病毒通用引物. VectorPrimer (F)Primer (R)抗性载体大小 (bp)EZ-TM13-47RV-MAmp+2984LZRS-RfALZRS-FLZRS-R11100p3xFLAGMV14CMV30 (834bp)CMV24 ... WebbLower panel revealed that pHis2.1 vector encompassing FL, ∆1, or ∆2 promoter region of the ANAC003 gene was amplified, respectively by using the primer set located at the … grand forks public school directory

质粒表达载体pCMV-BD载体 pCMV-AD载体 pBD-p53载体 pAD …

15 questions with answers in YEAST ONE-HYBRID Scientific …

Webb12 apr. 2024 · 目的：筛选与启动子pHIS2-A互作的蛋白。. 方法：以启动子A构建到酵母单杂交启动子载体pHIS2，文库质粒为pGADT7-中山杉文库。. 材料：. 1．启动子克 … Webb27 mars 2024 · 筛选含有报道质粒的酵母细胞具体过程为：合成靶序列；将靶序列插入pHIS2载体的多克隆位点；一般为将0.1mg pHIS2质粒用EcoRⅠ和SacⅠ进行完全双酶 … chinese culture and familyhttp://www.yrgene.com/documents/vector/matchmaker_library_constructionscreening_kits_user_manual.pdf chinese culture and contemporary china

"Webb8 juli 2024 · pHis2 编号载体名称北京华越洋生物VECT2110 pHis2 pHis2 载体基本信息出品公司: Clontech 载体名称: pHis2 质粒类型: 酵母单杂交载体高拷贝/低拷贝: -- 启动子: -- 克隆方法: 多克隆位点，限制性内切酶载体大小: 7207bp 5' 测序引物及序列: -- 3' 测序引物及序列: -- 载体标签: -- 载体抗性: 卡纳霉素筛选标记: -- 备注: 含HIS3 报告基因产品目录号: -- … " - Phis2.1 vector

Phis2.1 vector

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http://www.honorgene.com/product_list/carrier_library/123112.html Webb质粒简介 pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with …

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Webb13 juni 2015 · GTP1-GTP8 indicates the pGAD-OsGRAS23 plus pHIS2.1-cis (promoters of Os07g0162450, Os01g0537250, Os07g0638400 ... the promoter sequences were cloned into the yeast expression vector pHIS2.1 between the SmaI and EcoRI sites. The constructs were co-transformed into the yeast Y187 (MATa; ura3–52; his3–200; … WebbVectors in Use. N or C-terminal His tag. Parallel vectors 1 & 3 contain N-terminal His (pET22), MBP (pMal) and GST (pGex) with similar MCS for easy cloning. Parallel vector 2 contains N and C-terminal His (pET22), …

Webb6 feb. 2024 · Lower panel revealed that pHis2.1 vector encompassing FL, Δ1, or Δ2 promoter region of the ANAC003 gene was amplified, respectively by using the primer … Webb方法通过克隆 MaAQP1 的启动子，将启动子构建到pHIS2诱饵质粒上，转化酵母菌构建诱饵表达载体，同时构建干旱胁迫的香蕉（ Musa acuminat L. AAA group cv. Brazilian）cDNA文库。结果克隆获得1 362 bp的启动子序列，通过分析其顺式作用元件，结果显示启动子序列中共有72个顺式作用元件，包括了TATA-box和CAAT-box核心元件，ABA响应元件 …

Webb19 sep. 2024 · The pHIS2 vector carries the TRP1 nutritional marker for selection in yeast. The transformed yeast cells were cultured on SD/-Trp-Leu medium and SD/-Trp-His-Leu medium supplemented with 10 mM or 30 mM 3-amino … Webb21 mars 2024 · 其中，香豆素（1,2-苯并吡喃酮）的生理浓度足以抑制苹果枝在hk组织中的生长，外源施用可以提高lk苹果枝条对苹果枝的抗性。过表达β-葡萄糖苷酶40（MdBGLU40）的转基因苹果愈伤组织编码香豆素合成酶，含有更高水平的香豆素，即使在LK条件下也表现出对苹果枯萎病的高抗性。

WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article …

WebbBackground Rice transcription regulator OsWRKY13 influences the functioning of more than 500 genes in multiple signalling pathways, with roles in disease resistance, redox homeostasis, abiotic stress responses, and development. Results To determine chinese culture and nursingWebb1.一袋培养基干粉倒入三角瓶或培养瓶中，加0.5L去离子水中溶解（琼脂冷水不溶）。 2.无调整pH值，121℃高压灭菌15min。 3.液体培养基封好口避光、室温储存备用。 4.固体 … grand forks public recordshttp://www.honorgene.com/uploads/files/202411/6181dfe1813d2.pdf grand forks public school districthttp://www.honorgene.com/uploads/files/202411/6181dfe1813d2.pdf chinese culture and languageWebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … chinese culture and meWebb27 apr. 2024 · As with the positive control, yeast cells co-transformed with AD-PpPIF8 and proPpCHS-pHIS2.1 vectors could also grow on both selective mediums. However, the negative control yeast cells co-transformed with empty AD and proPpCHS -pHIS2.1 vectors could only grow on SD/−Leu−Trp medium ( Figure 11 F). chinese culture and healthhttp://www.zoonbio.com/molecular/yeast-one-hybrid-procedure.html grand forks public library jobs